Differential expression of NBS-LRR-encoding genes in the root transcriptomes of two Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis

Alex V. Kochetov, Anastasiya Y. Glagoleva, Kseniya V. Strygina, Elena K. Khlestkina, Sophia V. Gerasimova, Salmaz M. Ibragimova, Natalja V. Shatskaya, Gennady V. Vasilyev, Dmitry A. Afonnikov, Nikolay A. Shmakov, Olga Y. Antonova, Tatyana A. Gavrilenko, Natalia V. Alpatyeva, Alexander Khiutti, Olga S. Afanasenko

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Background: The characterization of major resistance genes (R genes) in the potato remains an important task for molecular breeding. However, R genes are rapidly evolving and frequently occur in genomes as clusters with complex structures, and their precise mapping and identification are complicated and time consuming. Results: Comparative analysis of root transcriptomes of Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis revealed a number of differentially expressed genes. However, compiling a list of candidate R genes for further segregation analysis was hampered by their scarce annotation. Nevertheless, combination of transcriptomic analysis with data on predicted potato NBS-LRR-encoding genes considerably improved the quality of the results and provided a reasonable number of candidate genes that provide S. phureja with strong resistance to the potato golden cyst nematode. Conclusion: Combination of comparative analyses of tissue-specific transcriptomes in resistant and susceptible genotypes may be used as an approach for the rapid identification of candidate potato R genes for co-segregation analysis and may be used in parallel with more sophisticated studies based on genome resequencing.

Original languageEnglish
Article number251
Pages (from-to)251
Number of pages10
JournalBMC Plant Biology
Volume17
Issue numberSuppl 2
DOIs
Publication statusPublished - 28 Dec 2017

Keywords

  • Globodera rostochiensis
  • NBS-LRR genes
  • Resistance
  • Solanum phureja
  • Gene Expression Regulation, Plant/genetics
  • Genes, Plant/genetics
  • Animals
  • Tylenchoidea/physiology
  • Solanum/genetics
  • Disease Resistance/genetics
  • Transcriptome/genetics
  • Plant Roots/metabolism
  • NICOTIANA-BENTHAMIANA
  • POTATO
  • HIGH-RESOLUTION MAP
  • NEMATODE
  • CONFERRING RESISTANCE
  • ENHANCES DISEASE RESISTANCE
  • RNA-SEQ
  • BROAD-SPECTRUM RESISTANCE
  • CANDIDATE GENE
  • HYPERSENSITIVE RESPONSE

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