Determination of the Thermodynamic Parameters of DNA Double Helix Unwinding with the Help of Mechanical Methods

Nina N. Kurus, Fedor N. Dultsev

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

For the first time, rupture event scanning (REVS) procedure based on quartz crystal microbalance (QCM) and involving only mechanical action was used to determine the height of the energy barrier for dsDNA unwinding. Melting point was determined with the help of this procedure. To determine the thermodynamic parameters including enthalpy, DNA denaturation was represented as a unimolecular process. This allowed us to recover the energy profiles from the experimental data obtained by force measurements at different scanning times (reaction times) for different temperatures. The thus obtained results were compared with the data obtained with the help of another mechanical method, namely, atomic force microscopy. The mechanism of DNA unwinding in QCM-based experiments through the unzipping mode, as proposed by us in previous works, was confirmed. Thus, we demonstrated that REVS procedure may be used to assess the thermodynamic parameters of dsDNA unwinding.

Original languageEnglish
Pages (from-to)2793-2797
Number of pages5
JournalACS Omega
Volume3
Issue number3
DOIs
Publication statusPublished - 31 Mar 2018

Keywords

  • ADHESION
  • BOND RUPTURE FORCES
  • DUPLEX
  • DYNAMIC FORCE SPECTROSCOPY
  • ENERGY LANDSCAPES
  • EVENTS
  • MICROSCOPY
  • MISMATCHES
  • STRANDS
  • STRENGTH

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