Cytokines production by blood immune cells in patients of different age groups with invasive ductal carcinoma of no special type and lymphatic metastases

K. I. Davletova, E. S. Mikhaylova, N. A. Varaksin, I. P. Zhurakovsky, A. V. Proskura, S. V. Sidorov, A. I. Autenshlyus

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to evaluate production of cytokines by the blood immune cells in patients of different age groups with invasive non-specified type mammary ductal carcinoma, with or without metastases. Production of cytokines by periphery blood immunocompetent cells, either spontaneous and stimulated with polyclonal activators, was assessed in 82 patients with invasive mammary ductal carcinoma. The concentrations of IL-6, IL-8, IL-10, IL-17, IL-18, IL-1β, IL-1ra, TNFα, IFNγ, G-CSF, GM-CSF, VEGF 'Greek Passage' MCP-1 were determined by solid-phase enzyme-linked immunosorbent assay. The patients were divided into 2 age groups: the first group consisted of 48 patients from 45 to 60 years old; the second group consisted of 34 older patients (61 to 75 years old). Metastases in local lymph nodes were documented in twenty patients from the first group and nine patients from the second group. The younger patients (45-60 years old) with metastases in local lymph nodes showed higher polyclonal activation index of IL-4 and IL-1ra production, when compared to the patients without lymphatic metastases. As for the older patients (61 to 75 years old), their polyclonal activation index of IL-6, IL-8, IL-1ra, G-CFS, GM-CSF production was significantly lower in cases of local lymph nodes metastases. The latter was due to higher level of spontaneous production, which suppressed the influence of polyclonal activators. The index of polyclonal activation upon production of cytokines in patients with lymphatic metastases was shown to be significantly higher for the age group of 45 to 60 years than in the age group of 61 to 75 years old. This fact suggested a highly stimulating effect of polyclonal activators in patients younger than 60 years. It was found that correlation between the index of polyclonal activation of cytokine production, and the indexes of ER, PR, HER2/NEU and Ki-67 receptor expression, which are used for the determination of molecular genetic subtype of the tumor, differ significantly for the distinct age groups.

Original languageEnglish
Pages (from-to)1115-1126
Number of pages12
JournalMedical Immunology (Russia)
Volume21
Issue number6
DOIs
Publication statusPublished - 1 Nov 2019

Keywords

  • Blood immune cells
  • Breast cancer
  • Cytokines
  • Invasive ductal carcinoma of no special type
  • Lymph nodes metastasis
  • Polyclonal activators

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