Comparative Metabolomic Profiling of Rat Embryonic and Induced Pluripotent Stem Cells

Vladimir V. Sherstyuk, Lyudmila V. Yanshole, Ekaterina A. Zelentsova, Arsenty D. Melnikov, Sergey P. Medvedev, Yuri P. Tsentalovich, Suren M. Zakian

Research output: Contribution to journalArticlepeer-review


Metabolomic profiles of somatic cells, embryonic stem cells (ESCs), and induced pluripotent stem cells (iPSCs) reflect their metabolic phenotypes. The comparative study of metabolomes of these cells is important for understanding the differences in metabolism between somatic and pluripotent cells, and also the possible differences between ESCs and iPSCs. Here, we performed for the first time the metabolomic analysis of rat ESCs, iPSCs, and embryonic fibroblasts (EFs) at both quantitative and semi-quantitative levels using NMR spectroscopy and liquid chromatography with mass spectrometric detection, respectively. The total of 106 metabolites has been identified, and the concentrations of 51 compounds have been measured. It is found that the reprogramming of rat EFs into iPSCs affects virtually all metabolic pathways and causes drastic changes in the cell metabolomic profile. The difference between ESCs and iPSCs is much less pronounced: the concentrations of the majority of metabolites in ESCs and iPSCs are similar, and significant differences were observed for only several compounds, including adenosine, cysteic acid, glycerophosphoglycerol, inositol phosphate, glucose, myo-inositol, phosphoserine, xanthosine, guanosine. The observed differences between the metabolomic compositions of ESCs and iPSCs do not influence the pluripotent ability of iPSCs. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)1256-1265
Number of pages10
JournalStem Cell Reviews and Reports
Issue number6
Publication statusPublished - Dec 2020


  • Embryonic stem cells
  • Induced pluripotent stem cells
  • LC-MS
  • Metabolomics
  • NMR
  • Pluripotency
  • Rat
  • Reprogramming

Fingerprint Dive into the research topics of 'Comparative Metabolomic Profiling of Rat Embryonic and Induced Pluripotent Stem Cells'. Together they form a unique fingerprint.

Cite this