Communication: Alamethicin can capture lipid-like molecules in the membrane

Ekaterina F. Afanasyeva, Victoria N. Syryamina, Sergei A. Dzuba

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Alamethicin (Alm) is a 19-mer antimicrobial peptide produced by fungus Trichoderma viride. Above a threshold concentration, Alm forms pores across the membrane, providing a mechanism of its antimicrobial action. Here we show that at a small concentration which is below the threshold value, Alm participates in formation of nanoscale lipid-mediated clusters of guest lipid-like molecules in the membrane. These results are obtained by electron spin echo (ESE) technique - a pulsed version of electron paramagnetic resonance - on spin-labeled stearic acid in a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine bilayer with Alm added at 1/200 peptide-to-lipid ratio. ESE decay measurements are interpreted assuming that stearic acid molecules in the membrane are assembling around the Alm molecule. One may suggest that this Alm capturing effect on the guest lipid-like molecules could be important for the peptide antimicrobial action.

Original languageEnglish
Article number011103
Pages (from-to)011103
Number of pages4
JournalJournal of Chemical Physics
Volume146
Issue number1
DOIs
Publication statusPublished - 7 Jan 2017

Keywords

  • Alamethicin/chemistry
  • Amino Acid Sequence
  • Cell Membrane/drug effects
  • Electron Spin Resonance Spectroscopy
  • Phosphatidylcholines/chemistry
  • Temperature
  • CHOLESTEROL
  • MECHANISM
  • ANTIMICROBIAL PEPTIDES
  • BILAYER
  • DYNAMICS SIMULATIONS
  • RESONANCE
  • TEMPERATURE
  • ORIENTATION
  • MELITTIN
  • SPIN LABELS

Fingerprint Dive into the research topics of 'Communication: Alamethicin can capture lipid-like molecules in the membrane'. Together they form a unique fingerprint.

Cite this