Co-Expression of Membrane-Bound Tumor Necrosis Factor-Alpha Receptor Types 1 and 2 by Tumor Cell Lines

Alina A. Alshevskaya, Irina Belomestnova, Julia A. Lopatnikova, Julia Zhukova, Irina Evsegneeva, Olga Koneva, Aleksander V. Karaulov, Sergey Vitalievich Sennikov

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Introduction: Density and co-expression of tumor necrosis factor (TNF) receptors may vary among cell populations. However, the role and potential of these changes remain unclear. This study aimed to determine the density of expression and co-expression of TNFR1/2 and the dose-dependent effect of soluble TNF on these parameters. Methods: Epithelial-like (HEp-2, K-562, MCF-7, ZR-75/1) and lymphoblast-like (MOLT-4, HL-60, Raji, RPMI-8226, IM-9) cell lines were characterized for co-expression of TNFR1/2 using a modified flow cytometry protocol. The dose-dependent effects of rhTNF on TNF receptor expression in these lines were studied. Results: This study reports a protocol for the simultaneous quantitative evaluation of the of TNF receptor number and co-expression of membrane-bound TNFR1/2. Cells within one tumor cell line were found to differ regarding their expression of type 1 and 2 TNFα receptors; simultaneously, cells with all 4 variants of co-expression may be present in culture. Conclusion: We demonstrated a dose-dependent effect of TNF on changes in the expression of TNFR1/2 by the percentage of positive cells and by the number of receptors, which may be used to control TNF-mediated processes in target cells.

Original languageEnglish
Pages (from-to)249-256
Number of pages8
JournalInternational Archives of Allergy and Immunology
Issue number4
Publication statusPublished - 1 Apr 2020


  • Cellular immunology
  • Cytokine receptors expression
  • Immune regulation
  • TNFα


Dive into the research topics of 'Co-Expression of Membrane-Bound Tumor Necrosis Factor-Alpha Receptor Types 1 and 2 by Tumor Cell Lines'. Together they form a unique fingerprint.

Cite this