Chondroitin sulfate content and decorin expression in glioblastoma are associated with proliferative activity of glioma cells and disease prognosis

Alexandra Y. Tsidulko, Galina M. Kazanskaya, Alexander M. Volkov, Anastasia V. Suhovskih, Roman S. Kiselev, Vyacheslav V. Kobozev, Alexei S. Gaytan, Alexei L. Krivoshapkin, Svetlana V. Aidagulova, Elvira V. Grigorieva

Research output: Contribution to journalArticle

Abstract

Chondroitin sulfate proteoglycans (CSPGs) are important components of brain extracellular matrix (ECM), although their contribution in gliomagenesis remains underinvestigated. Here, both chondroitin sulfate (CS) content/distribution and expression of a number of CSPG core proteins were studied in glioblastoma multiforme (GBM) tumours with different prognosis (n = 40) using immunohistochemistry and RT-PCR analysis. Survival rates for clinically different patient groups were compared using the Kaplan-Meier analysis and univariate Cox model. CS content was increased in 60–65% of studied GBM tumours and distributed heterogeneously, mainly at perinecrotic and perivascular zones rather than tumour cells with specific morphology. CS accumulation, especially in the tumour extracellular matrix, was positively associated with the proliferative activity of GBM cells according to theKi67 index (p < 0.01) but revealed no significant association with age or sex of the patients, tumour localisation, relapse or disease outcome. The increase in CS content in GBM tumours was accompanied by upregulation of decorin (1.5-fold), biglycan (3-fold) and serglycin (2-fold) expression (p < 0.05), while only decorin expression level was negatively associated with the overall survival rate of the GBM patients (p < 0.05). These results demonstrate a contribution of CS to high intratumoural heterogeneity of GBM and suggest CS content and decorin expression for further investigation as potential microenvironmental glycomarkers/targets for GBM diagnostics and treatment.

Original languageEnglish
Pages (from-to)147-155
Number of pages9
JournalCell and Tissue Research
Volume379
Issue number1
Early online date26 Nov 2019
DOIs
Publication statusPublished - 1 Jan 2020

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Keywords

  • Chondroitin sulfate proteoglycan
  • Decorin
  • Extracellular matrix
  • Glioblastoma multiforme
  • Tumour microenvironment

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