TY - JOUR
T1 - Chelidonic Acid and Its Derivatives from Saussurea Controversa
T2 - Isolation, Structural Elucidation and Influence on the Osteogenic Differentiation of Multipotent Mesenchymal Stromal Cells In Vitro
AU - Avdeeva, Elena
AU - Shults, Elvira
AU - Rybalova, Tatyana
AU - Reshetov, Yaroslav
AU - Porokhova, Ekaterina
AU - Sukhodolo, Irina
AU - Litvinova, Larisa
AU - Shupletsova, Valeria
AU - Khaziakhmatova, Olga
AU - Khlusov, Igor
AU - Guryev, Artem
AU - Belousov, Mikhail
N1 - Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2019/5/16
Y1 - 2019/5/16
N2 - 4-oxo-4H-pyran-2.6-dicarboxylic acid (chelidonic acid, ChA) in the native state and in the complex with calcium [Ca(ChA)(H2O)3], named saucalchelin (CaChA), was isolated from the extract of Saussurea controversa leaves for the first time for the Asteraceae family. The structure of ChA was determined by NMR, MS and confirmed by X-ray analysis of its monomethyl ester, and CaChA was described by IR, ICP-MS, CHN analysis. The yield of ChA and CaChA was 45 mg/g and 70 mg/g of extract, respectively. The osteogenic activity of ChA, n-monobutyl ester of chelidonic acid, and CaChA has been studied in vitro in a 21-day culture of human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs) in a standard nutrient medium without osteogenic supplements. CaChA significantly stimulated the growth of cell mass and differentiation of hAMMSCs into osteoblasts with subsequent mineralization of the culture and it may be a promising substance for accelerating bone tissue regeneration and engineering.
AB - 4-oxo-4H-pyran-2.6-dicarboxylic acid (chelidonic acid, ChA) in the native state and in the complex with calcium [Ca(ChA)(H2O)3], named saucalchelin (CaChA), was isolated from the extract of Saussurea controversa leaves for the first time for the Asteraceae family. The structure of ChA was determined by NMR, MS and confirmed by X-ray analysis of its monomethyl ester, and CaChA was described by IR, ICP-MS, CHN analysis. The yield of ChA and CaChA was 45 mg/g and 70 mg/g of extract, respectively. The osteogenic activity of ChA, n-monobutyl ester of chelidonic acid, and CaChA has been studied in vitro in a 21-day culture of human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs) in a standard nutrient medium without osteogenic supplements. CaChA significantly stimulated the growth of cell mass and differentiation of hAMMSCs into osteoblasts with subsequent mineralization of the culture and it may be a promising substance for accelerating bone tissue regeneration and engineering.
KW - 4-oxo-4H-pyran-2.6-dicarboxylic acid esters
KW - chelidonic acid
KW - human multipotent mesenchymal stromal cells
KW - Saussurea controversa DC (Asteraceae)
KW - X-ray diffraction analysis
KW - INTERNATIONAL-SOCIETY
KW - COMPLEXES
KW - 4-oxo-4H-pyran-2
KW - 6-dicarboxylic acid esters
KW - ADIPOSE-TISSUE
KW - Human multipotent mesenchymal stromal cells
KW - Chelidonic acid
UR - http://www.scopus.com/inward/record.url?scp=85066234373&partnerID=8YFLogxK
U2 - 10.3390/biom9050189
DO - 10.3390/biom9050189
M3 - Article
C2 - 31100934
AN - SCOPUS:85066234373
VL - 9
JO - Biomolecules
JF - Biomolecules
SN - 2218-273X
IS - 5
M1 - 189
ER -