Biological Studies of New Implant Materials Based on Carbon and Polymer Carriers with Film Heterostructures Containing Noble Metals

Svetlana I. Dorovskikh, Evgeniia S. Vikulova, David S. Sergeevichev, Tatiana Ya Guselnikova, Alexander A. Zheravin, Dmitriy A. Nasimov, Maria B. Vasilieva, Elena V. Chepeleva, Anatoly I. Saprykin, Tamara V. Basova, Natalya B. Morozova

Research output: Contribution to journalArticlepeer-review

Abstract

This paper presents pioneering results on the evaluation of noble metal film hetero-structures to improve some functional characteristics of carbon-based implant materials: carbon-composite material (CCM) and carbon-fiber-reinforced polyetheretherketone (CFR-PEEK). Metal-organic chemical vapor deposition (MOCVD) was successfully applied to the deposition of Ir, Pt, and PtIr films on these carriers. A noble metal layer as thin as 1 µm provided clear X-ray imaging of 1–2.5 mm thick CFR-PEEK samples. The coated and pristine CCM and CFR-PEEK samples were further surface-modified with Au and Ag nanoparticles (NPs) through MOCVD and physical vapor deposition (PVD) processes, respectively. The composition and microstructural features, the NPs sizes, and surface concentrations were determined. In vitro biological studies included tests for cytotoxicity and antibacterial properties. A series of samples were selected for subcutaneous implantation in rats (up to 3 months) and histological studies. The bimetallic PtIr-based heterostructures showed no cytotoxicity in vitro, but were less biocompatible due to a dense two-layered fibrous capsule. AuNP heterostructures on CFR-PEEK promoted cell proliferation in vitro and exhibited a strong inhibition of bacterial growth (p < 0.05) and high in vitro biocompatibility, especially Au/Ir structures. AgNP heterostructures showed a more pronounced antibacterial effect, while their in vivo biocompatibility was better than that of the pristine CFR-PEEK, but worse than that of AuNP heterostructures.

Original languageEnglish
Article number2230
JournalBiomedicines
Volume10
Issue number9
DOIs
Publication statusPublished - Sep 2022

Keywords

  • antibacterial studies
  • carbon
  • chemical vapor deposition
  • cytological studies
  • histological studies
  • polymer
  • precious metals
  • thin films and nanoparticles

OECD FOS+WOS

  • 1.06 BIOLOGICAL SCIENCES

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