Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus

Daria Prokhorova, Natalya Zhukova, Anna Lemza, Mariia Sergeeva, Rinat Amirkhanov, Grigory Stepanov

Research output: Contribution to journalReview articlepeer-review

Abstract

Influenza A virus (IAV) causes a respiratory infection that affects millions of people of different age groups and can lead to acute respiratory distress syndrome. Currently, host genes, receptors, and other cellular components critical for IAV replication are actively studied. One of the most convenient and accessible genome-editing tools to facilitate these studies is the CRISPR/Cas9 system. This tool allows for regulating the expression of both viral and host cell genes to enhance or impair viral entry and replication. This review considers the effect of the genome editing system on specific target genes in cells (human and chicken) in terms of subsequent changes in the influenza virus life cycle and the efficiency of virus particle production.

Original languageEnglish
Article number437
JournalViruses
Volume14
Issue number2
DOIs
Publication statusPublished - Feb 2022

Keywords

  • Cell receptors
  • Cellular immune response
  • CRISPR/Cas9
  • CRISPR/Cas9 genome screening
  • Influenza virus
  • RNA processing factors
  • Cell Line
  • Humans
  • Gene Editing/methods
  • Virus Internalization
  • CRISPR-Cas Systems/genetics
  • Influenza A virus/genetics
  • Animals
  • Virus Replication
  • Chickens
  • Immunity, Cellular

OECD FOS+WOS

  • 1.06.ZE VIROLOGY

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