Antisense Oligonucleotides Immobilized on Silicon-Organic Nanoparticles as a Tool for Prolonged Correction of Hypertensive States

A. S. Levina, M. N. Repkova, L. O. Klimov, M. A. Ryazanova, A. L. Markel, V. F. Zarytova

Research output: Contribution to journalArticlepeer-review

Abstract

We propose an original method for controlling BP by administration of Si~ODN nanocomposites containing antisense oligonucleotides fixed on silicon-organic nanoparticles. ODN in nanocomposites are targeted to mRNA of the genes encoding angiotensin-converting enzyme (ACE1) and type 1 angiotensin-II receptor (AT1A). The experiments were performed on hypertensive ISIAH rats, a genetic model of hypertension. Single inhalation or intraperitoneal administration of the nanocomposites targeted to ACE1 mRNA or ATA1 mRNA, respectively, led to a pronounced decrease (by ~30 mm Hg) in systolic BP in ISIAH rats over a week. The use of scrambled ODN in the nanocomposites had no effect. A decrease in the expression of ACE1 and AT1A genes under the effect of the corresponding antisense ODN was demonstrated, which attested to directed effect of the test preparations.

Original languageEnglish
Pages (from-to)116-119
Number of pages4
JournalBulletin of Experimental Biology and Medicine
Volume167
Issue number1
DOIs
Publication statusPublished - 15 May 2019

Keywords

  • antisense oligonucleotides
  • arterial hypertension
  • nanocomposites
  • silicon-organic nanoparticles
  • RNA

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