Analysis of new charge-neutral DNA/RNA analogues phosphoryl guanidine oligonucleotides (PGO) by gel electrophoresis

Alesya Fokina, Meiling Wang, Anna Ilyina, Kristina Klabenkova, Ekaterina Burakova, Boris Chelobanov, Dmitry Stetsenko

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Analysis and isolation of new charge-neutral phosphoryl guanidine oligonucleotides (PGO) by vertical slab electrophoresis were tested at different pH values (3–11) or in the presence of SDS as a micelle-forming agent. The most convenient way to analyze and purify phosphoryl guanidine oligonucleotides was by denaturing PAGE (8 M urea) at pH 3. The mobility of PGO is dependent on their A + C content. To analyze PGO containing only G, T or U, denaturing PAGE at pH 11 can be used, although the conditions need to be optimized. Bands were visualized by UV shadowing or Coomassie Brilliant Blue staining.

Original languageEnglish
Pages (from-to)9-11
Number of pages3
JournalAnalytical Biochemistry
Volume555
DOIs
Publication statusPublished - 15 Aug 2018

Keywords

  • DNA/analysis
  • Electrophoresis, Polyacrylamide Gel
  • Guanidine/chemistry
  • Oligonucleotides/analysis
  • RNA/analysis

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