Analysis of new charge-neutral DNA/RNA analogues phosphoryl guanidine oligonucleotides (PGO) by gel electrophoresis

Alesya Fokina; Meiling Wang; Anna Ilyina; Kristina Klabenkova; Ekaterina Burakova; Boris Chelobanov; Dmitry Stetsenko

doi:10.1016/j.ab.2018.05.027

Analysis of new charge-neutral DNA/RNA analogues phosphoryl guanidine oligonucleotides (PGO) by gel electrophoresis

Alesya Fokina, Meiling Wang, Anna Ilyina, Kristina Klabenkova, Ekaterina Burakova, Boris Chelobanov, Dmitry Stetsenko

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Analysis and isolation of new charge-neutral phosphoryl guanidine oligonucleotides (PGO) by vertical slab electrophoresis were tested at different pH values (3–11) or in the presence of SDS as a micelle-forming agent. The most convenient way to analyze and purify phosphoryl guanidine oligonucleotides was by denaturing PAGE (8 M urea) at pH 3. The mobility of PGO is dependent on their A + C content. To analyze PGO containing only G, T or U, denaturing PAGE at pH 11 can be used, although the conditions need to be optimized. Bands were visualized by UV shadowing or Coomassie Brilliant Blue staining.

Original language	English
Pages (from-to)	9-11
Number of pages	3
Journal	Analytical Biochemistry
Volume	555
DOIs	https://doi.org/10.1016/j.ab.2018.05.027
Publication status	Published - 15 Aug 2018

Keywords

DNA/analysis
Electrophoresis, Polyacrylamide Gel
Guanidine/chemistry
Oligonucleotides/analysis
RNA/analysis

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title = "Analysis of new charge-neutral DNA/RNA analogues phosphoryl guanidine oligonucleotides (PGO) by gel electrophoresis",

abstract = "Analysis and isolation of new charge-neutral phosphoryl guanidine oligonucleotides (PGO) by vertical slab electrophoresis were tested at different pH values (3–11) or in the presence of SDS as a micelle-forming agent. The most convenient way to analyze and purify phosphoryl guanidine oligonucleotides was by denaturing PAGE (8 M urea) at pH 3. The mobility of PGO is dependent on their A + C content. To analyze PGO containing only G, T or U, denaturing PAGE at pH 11 can be used, although the conditions need to be optimized. Bands were visualized by UV shadowing or Coomassie Brilliant Blue staining.",

keywords = "DNA/analysis, Electrophoresis, Polyacrylamide Gel, Guanidine/chemistry, Oligonucleotides/analysis, RNA/analysis",

author = "Alesya Fokina and Meiling Wang and Anna Ilyina and Kristina Klabenkova and Ekaterina Burakova and Boris Chelobanov and Dmitry Stetsenko",

note = "Copyright {\textcopyright} 2018. Published by Elsevier Inc.",

year = "2018",

month = aug,

day = "15",

doi = "10.1016/j.ab.2018.05.027",

language = "English",

volume = "555",

pages = "9--11",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

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Analysis of new charge-neutral DNA/RNA analogues phosphoryl guanidine oligonucleotides (PGO) by gel electrophoresis. / Fokina, Alesya; Wang, Meiling; Ilyina, Anna; Klabenkova, Kristina; Burakova, Ekaterina; Chelobanov, Boris ; Stetsenko, Dmitry.

In: Analytical Biochemistry, Vol. 555, 15.08.2018, p. 9-11.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Analysis of new charge-neutral DNA/RNA analogues phosphoryl guanidine oligonucleotides (PGO) by gel electrophoresis

AU - Fokina, Alesya

AU - Wang, Meiling

AU - Ilyina, Anna

AU - Klabenkova, Kristina

AU - Burakova, Ekaterina

AU - Chelobanov, Boris

AU - Stetsenko, Dmitry

PY - 2018/8/15

Y1 - 2018/8/15

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AB - Analysis and isolation of new charge-neutral phosphoryl guanidine oligonucleotides (PGO) by vertical slab electrophoresis were tested at different pH values (3–11) or in the presence of SDS as a micelle-forming agent. The most convenient way to analyze and purify phosphoryl guanidine oligonucleotides was by denaturing PAGE (8 M urea) at pH 3. The mobility of PGO is dependent on their A + C content. To analyze PGO containing only G, T or U, denaturing PAGE at pH 11 can be used, although the conditions need to be optimized. Bands were visualized by UV shadowing or Coomassie Brilliant Blue staining.

KW - DNA/analysis

KW - Electrophoresis, Polyacrylamide Gel

KW - Guanidine/chemistry

KW - Oligonucleotides/analysis

KW - RNA/analysis

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