Abstract
Analysis and isolation of new charge-neutral phosphoryl guanidine oligonucleotides (PGO) by vertical slab electrophoresis were tested at different pH values (3–11) or in the presence of SDS as a micelle-forming agent. The most convenient way to analyze and purify phosphoryl guanidine oligonucleotides was by denaturing PAGE (8 M urea) at pH 3. The mobility of PGO is dependent on their A + C content. To analyze PGO containing only G, T or U, denaturing PAGE at pH 11 can be used, although the conditions need to be optimized. Bands were visualized by UV shadowing or Coomassie Brilliant Blue staining.
Original language | English |
---|---|
Pages (from-to) | 9-11 |
Number of pages | 3 |
Journal | Analytical Biochemistry |
Volume | 555 |
DOIs | |
Publication status | Published - 15 Aug 2018 |
Keywords
- DNA/analysis
- Electrophoresis, Polyacrylamide Gel
- Guanidine/chemistry
- Oligonucleotides/analysis
- RNA/analysis