Analysis of in vitro activity of PSCA-specific CARs in the context of human NK cell line YT

Daria A. Matvienko, Sergey V. Kulemzin, Anna S. Smagina, Tatyana N. Belovezhets, Anton N. Chikaev, Olga Y. Volkova, Nikolay A. Chikaev, Olga A. Koval, Elena V. Kuligina, Alexandr V. Taranin, Andrey A. Gorchakov

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Comprehensive structural optimization of chimeric antigen receptors (CARs) is the key to their successful performance both in vitro and in vivo. In order to compare various CAR designs in an unified format, we took advantage of a lentiviral platform, where all CAR modules can be easily shuffled and tested for functionality. This platform was used to delineate the effects of various spacer regions on the function of a PSCA-specific CAR in the context of a human NK cell line, YT. We show that three CAR designs (IgG1-, CD8a-, and spacerless) perform similarly in vitro regardless of the length of the spacer region.

Original languageEnglish
Pages (from-to)70-77
Number of pages8
JournalCellular Therapy and Transplantation
Volume7
Issue number2
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • Adoptive immunotherapy
  • Chimeric antigen receptor (CAR)
  • Prostate cancer
  • Prostate stem cell antigen (PSCA)

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