A highly versatile automatized setup for quantitative measurements of PHIP enhancements

Alexey S. Kiryutin, Grit Sauer, Sara Hadjiali, Alexandra V. Yurkovskaya, Hergen Breitzke, Gerd Buntkowsky

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

The design and application of a versatile and inexpensive experimental extension to NMR spectrometers is described that allows to carry out highly reproducible PHIP experiments directly in the NMR sample tube, i.e. under PASADENA condition, followed by the detection of the NMR spectra of hyperpolarized products with high spectral resolution. Employing this high resolution it is feasible to study kinetic processes in the solution with high accuracy. As a practical example the dissolution of hydrogen gas in the liquid and the PHIP kinetics during the hydrogenation reaction of Fmoc-O-propargyl-L-tyrosine in acetone-d6 are monitored. The timing of the setup is fully controlled by the pulse-programmer of the NMR spectrometer. By flushing with an inert gas it is possible to efficiently quench the hydrogenation reaction in a controlled fashion and to detect the relaxation of hyperpolarization without a background reaction. The proposed design makes it possible to carry out PHIP experiments in an automatic mode and reliably determine the enhancement of polarized signals.

Original languageEnglish
Pages (from-to)26-36
Number of pages11
JournalJournal of Magnetic Resonance
Volume285
DOIs
Publication statusPublished - 1 Dec 2017

Keywords

  • Gas-dissolution
  • Hyperpolarization
  • Kinetics
  • Parahydrogen Induced Polarization
  • SIGNAL ENHANCEMENT
  • CONTRAST AGENTS
  • NUCLEAR-MAGNETIC-RESONANCE
  • SPIN HYPERPOLARIZATION
  • REVERSIBLE EXCHANGE
  • HYDROGEN
  • SPECTROSCOPY
  • SABRE
  • PARAHYDROGEN-INDUCED POLARIZATION
  • STATE NMR CHARACTERIZATION

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