5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches

D. S. Novopashina, O. A. Semikolenova, A. G. Venyaminova

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

Abstract

Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and 5′-bispyrene conjugates of oligo(2′-O-methylribonucleotides) and their application as probes for fluorescent detection of mismatches in RNA targets.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages45-56
Number of pages12
Volume2063
DOIs
Publication statusPublished - 1 Jan 2020

Publication series

NameMethods in Molecular Biology
Volume2063
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Excimeric and monomeric fluorescence
  • Mismatch detection
  • Oligo(2′-O-methylribonucleotides)
  • Pyrene conjugates
  • RNA

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