5'‑дезоксирибозофатлиазная активность апуриновой/апиримидиновой эндонуклеазы 1

Translated title of the contribution: 5'-Deoxyribose Phosphate Lyase Activity of Apurinic/Apyrimidinic Endonuclease 1

E. S. Ilina, O. I. Lavrik, S. N. Khodyreva

Research output: Contribution to journalArticlepeer-review


One of the most common DNA lesions is the appearance of apurinic/apyrimidinic (AP-) sites. The main repair pathway for AP sites is initiated by apurinic/apyrimidinic endonuclease 1 (APE1). Upon hydrolysis of the phosphodiester bond by this enzyme, a one nucleotide gap flanked by 3'-hydroxyl and 5'-deoxyribose phosphate groups on the 5'-side of the AP site is formed. After hydrolysis of the AP site, APE1 remains associated with the product for some time. In the present work, the ability of APE1 to form a product of covalent attachment of APE1 to DNA containing a gap with a 5'-deoxyribose phosphate residue was demonstrated. In addition, it was found that while in a complex with the product of hydrolysis of the AP site, APE1 exhibits 5'-deoxyribose phosphate lyase activity, cleaving off the 5'-deoxyribose phosphate residue. The presence of lyase activity in APE1 may be important for the repair of AP sites if there is a deficiency of, or mutations in DNA polymerase β, the main enzyme that removes the 5'-deoxyribose phosphate group.

Translated title of the contribution5'-Deoxyribose Phosphate Lyase Activity of Apurinic/Apyrimidinic Endonuclease 1
Original languageRussian
Pages (from-to)269-276
Number of pages8
JournalMolekuliarnaia biologiia
Issue number2
Publication statusPublished - 1 Mar 2021


  • affinity modification
  • apurinic/apyrimidinic endonuclease 1
  • apurinic/apyrimidinic sites
  • deoxyribose phosphate lyase activity
  • DNA repair



State classification of scientific and technological information

  • 34 BIOLOGY


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