Abstract
The protocol allows to define and characterize mitosis distribution patterns in the plant root meristem. The method does not require genetic markers, which makes it applicable to plants of different non-transgenic genotypes, including ecotypes, mutants, and non-model plant species. Computer analysis of the mitosis distribution in three dimensions with iRoCS Toolbox identifies statistically significant changes in proliferation activity within specific root tissues and cell lineages.
Original language | English |
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Pages (from-to) | 119-125 |
Number of pages | 7 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 2094 |
DOIs | |
Publication status | Published - 1 Jan 2020 |
Keywords
- Arabidopsis
- Cell cycle
- Cortex
- Endodermis
- Epidermis
- Image analysis
- Pericycle
- Proliferation
- Protophloem
- Protoxylem
- RAM