3D Analysis of Mitosis Distribution Pattern in the Plant Root Tip with iRoCS Toolbox

Viktoriya V. Lavrekha, Taras Pasternak, Klaus Palme, Victoria V. Mironova

Research output: Contribution to journalArticle


The protocol allows to define and characterize mitosis distribution patterns in the plant root meristem. The method does not require genetic markers, which makes it applicable to plants of different non-transgenic genotypes, including ecotypes, mutants, and non-model plant species. Computer analysis of the mitosis distribution in three dimensions with iRoCS Toolbox identifies statistically significant changes in proliferation activity within specific root tissues and cell lineages.

Original languageEnglish
Pages (from-to)119-125
Number of pages7
JournalMethods in molecular biology (Clifton, N.J.)
Publication statusPublished - 1 Jan 2020



  • Arabidopsis
  • Cell cycle
  • Cortex
  • Endodermis
  • Epidermis
  • Image analysis
  • Pericycle
  • Proliferation
  • Protophloem
  • Protoxylem
  • RAM

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