3D Analysis of Mitosis Distribution Pattern in the Plant Root Tip with iRoCS Toolbox

Viktoriya V. Lavrekha; Taras Pasternak; Klaus Palme; Victoria V. Mironova

doi:10.1007/978-1-0716-0183-9_13

3D Analysis of Mitosis Distribution Pattern in the Plant Root Tip with iRoCS Toolbox

Viktoriya V. Lavrekha, Taras Pasternak, Klaus Palme, Victoria V. Mironova

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The protocol allows to define and characterize mitosis distribution patterns in the plant root meristem. The method does not require genetic markers, which makes it applicable to plants of different non-transgenic genotypes, including ecotypes, mutants, and non-model plant species. Computer analysis of the mitosis distribution in three dimensions with iRoCS Toolbox identifies statistically significant changes in proliferation activity within specific root tissues and cell lineages.

Original language	English
Pages (from-to)	119-125
Number of pages	7
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2094
DOIs	https://doi.org/10.1007/978-1-0716-0183-9_13
Publication status	Published - 1 Jan 2020

Keywords

Arabidopsis
Cell cycle
Cortex
Endodermis
Epidermis
Image analysis
Pericycle
Proliferation
Protophloem
Protoxylem
RAM

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KW - Cell cycle

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KW - Endodermis

KW - Epidermis

KW - Image analysis

KW - Pericycle

KW - Proliferation

KW - Protophloem

KW - Protoxylem

KW - RAM

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