Экзосомы молока лошади: выделение, микроскопический и биохимический анализ, перспективы использования

Translated title of the contribution: Horse Milk Exosomes: Isolation, Microscopic and Biochemical Analysis, and Prospects of Use

S. E. SEDYKH, A. E. KULESHOVA, L. V. PURVINSH, E. E. BURKOVA, A. E. GRIGORIEVA, E. G. EVTUSHENKO, G. A. STEPANOV, E. I. RYABCHIKOVA, G. A. NEVINSKII

Research output: Contribution to journalArticlepeer-review

Abstract

Horse milk exosomes have been isolated and purified via the developed technology, and their microscopic and biochemical analyzes have been carried out. It was shown that the gel-filtration on the Ultrogel A4 resin can significantly reduce the amount of milk proteins co-isolated with exosomes. Methods for the isolation of nucleic acids from the preparations at various purification stages were proposed, and the content of nucleic acids in horse milk exosomes was analyzed. It was demonstrated that horse milk exosome preparations are not toxic to human cell cultures. The prospects of using horse milk exosomes for drug delivery into cell cultures are discussed.

Translated title of the contributionHorse Milk Exosomes: Isolation, Microscopic and Biochemical Analysis, and Prospects of Use
Original languageRussian
Pages (from-to)62-71
Number of pages10
JournalBiotekhnologiya
Volume36
Issue number5
DOIs
Publication statusPublished - 2020

Keywords

  • exosome isolation
  • exosomes
  • horse milk
  • nucleic acids

State classification of scientific and technological information

  • 34 BIOLOGY

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