Трансгенная клеточная линия с индуцируемой транскрипцией для исследования механизмов экспансии (CGG)n повторов

Translated title of the contribution: A transgenic cell line with inducible transcription for studying (CGG)n repeat expansion mechanisms

I. V. Grishchenko, A. A. Tulupov, Y. M. Rymareva, E. D. Petrovskiy, A. A. Savelov, A. M. Korostyshevskaya, Y. V. Maksimova, A. R. Shorina, E. M. Shitik, D. V. Yudkin

Research output: Contribution to journalArticlepeer-review

Abstract

There are more than 30 inherited human disorders connected with repeat expansion (myotonic dystrophy type I, Huntington's disease, Fragile X syndrome). Fragile X syndrome is the most common reason for inherited intellectual disability in the human population. The ways of the expansion development remain unclear. An important feature of expanded repeats is the ability to form stable alternative DNA secondary structures. There are hypotheses about the nature of repeat instability. It is proposed that these DNA secondary structures can block various stages of DNA metabolism processes, such as replication, repair and recombination and it is considered as the source of repeat instability. However, none of the hypotheses is fully conf irmed or is the only valid one. Here, an experimental system for studying (CGG)n repeat expansion associated with transcription and TCR-NER is proposed. It is noteworthy that the aberrations of transcription are a poorly studied mechanism of (CGG)n instability. However, the proposed systems take into account the contribution of other processes of DNA metabolism and, therefore, the developed systems are universal and applicable for various studies. Transgenic cell lines carrying a repeat of normal or premutant length under the control of an inducible promoter were established and a method for repeat instability quantif ication was developed. One type of the cell lines contains an exogenous repeat integrated into the genome by the Sleeping Beauty transposon; in another cell line, the vector is maintained as an episome due to the SV40 origin of replication. These experimental systems can serve for f inding the causes of instability and the development of therapeutic agents. In addition, a criterion was developed for the quantif ication of exogenous (CGG)n repeat instability in the transgenic cell lines' genome.

Translated title of the contributionA transgenic cell line with inducible transcription for studying (CGG)n repeat expansion mechanisms
Original languageRussian
Article number12
Pages (from-to)117-124
Number of pages8
JournalVavilovskii Zhurnal Genetiki i Selektsii
Volume25
Issue number1
DOIs
Publication statusPublished - Jan 2021

Keywords

  • Fragile X syndrome
  • Hereditary intellectual disability
  • Repeat expansion
  • Replication
  • Somatic instability
  • Transcription
  • Transgenic cell lines

OECD FOS+WOS

  • 3 MEDICAL AND HEALTH SCIENCES
  • 1.06 BIOLOGICAL SCIENCES
  • 1.06.KM GENETICS & HEREDITY

State classification of scientific and technological information

  • 34 BIOLOGY

Fingerprint

Dive into the research topics of 'A transgenic cell line with inducible transcription for studying (CGG)n repeat expansion mechanisms'. Together they form a unique fingerprint.

Cite this