Development of efficient antitumor modalities remains a priority of modern translation medicine. Adoptive transfer of autologous T cells expressing chimeric antigen receptors (CARs) specific for tumor-associated surface targets of B cells has demonstrated encouraging results in recent clinical trials. It is tempting therefore to adapt this approach to solid cancers and move it to universal/allogeneic formats. For this reason, it appears very attractive to use human NK cell lines as CAR carriers. Using lentiviral delivery, human NK cell line YT was modified to express a PSMA-specific CAR and to produce an anticancer peptide lactaptin. “Armored” CAR-YT-Lact cell line obtained shows cytotoxicity against PSMA-positive prostate adenocarcinoma cells. Accordingly, YT-Lact cells display a moderate and non-specific activity against PSMA-negative target cells, which may translate into a tighter control of tumor cell escape via antigen loss.
|Translated title of the contribution||Создание и анализ цитотоксической активности in vitro NK-клеточной линии человека с экспрессией PSMA-специфичного химерного антигенного рецептора и противоопухолевого агента лактаптина|
|Number of pages||5|
|Journal||Genes and Cells|
|Publication status||Published - 1 Jan 2018|
- NK cell lines
State classification of scientific and technological information
- 34 BIOLOGY