Analysis of in vitro cytotoxicity of human nk cell line co-expressing a psma-specific car and an antitumor agent lactaptin

T. N. Belovezhets; D. A. Matvienko; O. Y. Volkova; O. A. Koval; A. V. Tkachenko; E. V. Kuligina; A. V. Taranin; V. A. Richter

doi:10.23868/201811039

Analysis of in vitro cytotoxicity of human nk cell line co-expressing a psma-specific car and an antitumor agent lactaptin

T. N. Belovezhets, D. A. Matvienko, O. Y. Volkova, O. A. Koval, A. V. Tkachenko, E. V. Kuligina, A. V. Taranin, V. A. Richter

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Development of efficient antitumor modalities remains a priority of modern translation medicine. Adoptive transfer of autologous T cells expressing chimeric antigen receptors (CARs) specific for tumor-associated surface targets of B cells has demonstrated encouraging results in recent clinical trials. It is tempting therefore to adapt this approach to solid cancers and move it to universal/allogeneic formats. For this reason, it appears very attractive to use human NK cell lines as CAR carriers. Using lentiviral delivery, human NK cell line YT was modified to express a PSMA-specific CAR and to produce an anticancer peptide lactaptin. “Armored” CAR-YT-Lact cell line obtained shows cytotoxicity against PSMA-positive prostate adenocarcinoma cells. Accordingly, YT-Lact cells display a moderate and non-specific activity against PSMA-negative target cells, which may translate into a tighter control of tumor cell escape via antigen loss.

Translated title of the contribution	Создание и анализ цитотоксической активности in vitro NK-клеточной линии человека с экспрессией PSMA-специфичного химерного антигенного рецептора и противоопухолевого агента лактаптина
Original language	English
Pages (from-to)	89-93
Number of pages	5
Journal	Genes and Cells
Volume	13
Issue number	3
DOIs	https://doi.org/10.23868/201811039
Publication status	Published - 1 Jan 2018

Keywords

CAR
Lactaptin
NK cell lines
YT

State classification of scientific and technological information

34 BIOLOGY

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title = "Analysis of in vitro cytotoxicity of human nk cell line co-expressing a psma-specific car and an antitumor agent lactaptin",

abstract = "Development of efficient antitumor modalities remains a priority of modern translation medicine. Adoptive transfer of autologous T cells expressing chimeric antigen receptors (CARs) specific for tumor-associated surface targets of B cells has demonstrated encouraging results in recent clinical trials. It is tempting therefore to adapt this approach to solid cancers and move it to universal/allogeneic formats. For this reason, it appears very attractive to use human NK cell lines as CAR carriers. Using lentiviral delivery, human NK cell line YT was modified to express a PSMA-specific CAR and to produce an anticancer peptide lactaptin. “Armored” CAR-YT-Lact cell line obtained shows cytotoxicity against PSMA-positive prostate adenocarcinoma cells. Accordingly, YT-Lact cells display a moderate and non-specific activity against PSMA-negative target cells, which may translate into a tighter control of tumor cell escape via antigen loss.",

keywords = "CAR, Lactaptin, NK cell lines, YT",

author = "Belovezhets, {T. N.} and Matvienko, {D. A.} and Volkova, {O. Y.} and Koval, {O. A.} and Tkachenko, {A. V.} and Kuligina, {E. V.} and Taranin, {A. V.} and Richter, {V. A.}",

note = "Беловежец Т.Н., Матвиенко Д., Волкова О.Ю., Коваль О.А., Ткаченко А.В., Кулигина Е.В., Таранин А.В., Рихтер В. Создание и анализ цитотоксической активности in vitro NK-клеточной линии человека с экспрессией PSMA-специфичного химерного антигенного рецептора и противоопухолевого агента лактаптина // Гены и Клетки. - 2018. - Т. 13. - № 3. - С. 89-93",

year = "2018",

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volume = "13",

pages = "89--93",

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Analysis of in vitro cytotoxicity of human nk cell line co-expressing a psma-specific car and an antitumor agent lactaptin. / Belovezhets, T. N.; Matvienko, D. A.; Volkova, O. Y.; Koval, O. A.; Tkachenko, A. V.; Kuligina, E. V.; Taranin, A. V.; Richter, V. A.

In: Genes and Cells, Vol. 13, No. 3, 01.01.2018, p. 89-93.

Research output: Contribution to journal › Article › peer-review

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AU - Belovezhets, T. N.

AU - Matvienko, D. A.

AU - Volkova, O. Y.

AU - Koval, O. A.

AU - Tkachenko, A. V.

AU - Kuligina, E. V.

AU - Taranin, A. V.

AU - Richter, V. A.

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PY - 2018/1/1

Y1 - 2018/1/1

N2 - Development of efficient antitumor modalities remains a priority of modern translation medicine. Adoptive transfer of autologous T cells expressing chimeric antigen receptors (CARs) specific for tumor-associated surface targets of B cells has demonstrated encouraging results in recent clinical trials. It is tempting therefore to adapt this approach to solid cancers and move it to universal/allogeneic formats. For this reason, it appears very attractive to use human NK cell lines as CAR carriers. Using lentiviral delivery, human NK cell line YT was modified to express a PSMA-specific CAR and to produce an anticancer peptide lactaptin. “Armored” CAR-YT-Lact cell line obtained shows cytotoxicity against PSMA-positive prostate adenocarcinoma cells. Accordingly, YT-Lact cells display a moderate and non-specific activity against PSMA-negative target cells, which may translate into a tighter control of tumor cell escape via antigen loss.

AB - Development of efficient antitumor modalities remains a priority of modern translation medicine. Adoptive transfer of autologous T cells expressing chimeric antigen receptors (CARs) specific for tumor-associated surface targets of B cells has demonstrated encouraging results in recent clinical trials. It is tempting therefore to adapt this approach to solid cancers and move it to universal/allogeneic formats. For this reason, it appears very attractive to use human NK cell lines as CAR carriers. Using lentiviral delivery, human NK cell line YT was modified to express a PSMA-specific CAR and to produce an anticancer peptide lactaptin. “Armored” CAR-YT-Lact cell line obtained shows cytotoxicity against PSMA-positive prostate adenocarcinoma cells. Accordingly, YT-Lact cells display a moderate and non-specific activity against PSMA-negative target cells, which may translate into a tighter control of tumor cell escape via antigen loss.

KW - CAR

KW - Lactaptin

KW - NK cell lines

KW - YT

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