Analysis of in vitro cytotoxicity of human nk cell line co-expressing a psma-specific car and an antitumor agent lactaptin

T. N. Belovezhets, D. A. Matvienko, O. Y. Volkova, O. A. Koval, A. V. Tkachenko, E. V. Kuligina, A. V. Taranin, V. A. Richter

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Development of efficient antitumor modalities remains a priority of modern translation medicine. Adoptive transfer of autologous T cells expressing chimeric antigen receptors (CARs) specific for tumor-associated surface targets of B cells has demonstrated encouraging results in recent clinical trials. It is tempting therefore to adapt this approach to solid cancers and move it to universal/allogeneic formats. For this reason, it appears very attractive to use human NK cell lines as CAR carriers. Using lentiviral delivery, human NK cell line YT was modified to express a PSMA-specific CAR and to produce an anticancer peptide lactaptin. “Armored” CAR-YT-Lact cell line obtained shows cytotoxicity against PSMA-positive prostate adenocarcinoma cells. Accordingly, YT-Lact cells display a moderate and non-specific activity against PSMA-negative target cells, which may translate into a tighter control of tumor cell escape via antigen loss.

Translated title of the contributionСоздание и анализ цитотоксической активности in vitro NK-клеточной линии человека с экспрессией PSMA-специфичного химерного антигенного рецептора и противоопухолевого агента лактаптина
Original languageEnglish
Pages (from-to)89-93
Number of pages5
JournalGenes and Cells
Volume13
Issue number3
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • CAR
  • Lactaptin
  • NK cell lines
  • YT

State classification of scientific and technological information

  • 34 BIOLOGY

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