Translocation of Oligonucleotide–Oligosaccharide Complexes into Cells of the Brain

N. N. Dygalo; T. S. Kalinina; D. A. Lanshakov

doi:10.1134/S160767291802014X

Translocation of Oligonucleotide–Oligosaccharide Complexes into Cells of the Brain

N. N. Dygalo, T. S. Kalinina, D. A. Lanshakov

Physiology Section

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Irradiation of a mixture of oligonucleotides with dextran resulted in the formation of a complex that is detected by a decelerated migration of an irradiated sample in electrophoretic gel compared to a non-irradiated one. When injected into the brain of neonatal rats, the formed complex penetrated into the cells 3 times easier compared to the original oligonucleotide, thus indicating that the use of radiation crosslinking of oligonucleotides with oligosaccharides is promising to enhance the efficiency of delivery of gene-targeted oligonucleotide drugs into cells.

Translated title of the contribution	Поступление комплексов олигонуклеотидов с олигосахаридами в клетки головного мозга
Original language	English
Article number	15
Pages (from-to)	108-110
Number of pages	3
Journal	Doklady Biochemistry and Biophysics
Volume	479
Issue number	1
DOIs	https://doi.org/10.1134/S160767291802014X
Publication status	Published - 1 Mar 2018

Keywords

Animals
Biological Transport
Brain/metabolism
Oligonucleotides/metabolism
Oligosaccharides/metabolism
Rats

OECD FOS+WOS

1.06.CQ BIOCHEMISTRY & MOLECULAR BIOLOGY
1.06.DA BIOPHYSICS

State classification of scientific and technological information

34 BIOLOGY

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10.1134/S160767291802014X

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title = "Translocation of Oligonucleotide–Oligosaccharide Complexes into Cells of the Brain",

abstract = "Irradiation of a mixture of oligonucleotides with dextran resulted in the formation of a complex that is detected by a decelerated migration of an irradiated sample in electrophoretic gel compared to a non-irradiated one. When injected into the brain of neonatal rats, the formed complex penetrated into the cells 3 times easier compared to the original oligonucleotide, thus indicating that the use of radiation crosslinking of oligonucleotides with oligosaccharides is promising to enhance the efficiency of delivery of gene-targeted oligonucleotide drugs into cells.",

keywords = "Animals, Biological Transport, Brain/metabolism, Oligonucleotides/metabolism, Oligosaccharides/metabolism, Rats",

author = "Dygalo, {N. N.} and Kalinina, {T. S.} and Lanshakov, {D. A.}",

note = "Funding Information: We are grateful to M.V. Korobeinikov for irradiation of samples in the accelerator. This study was supported by a program of the Russian Academy of Sciences (project no. 0324-2016-0011). Publisher Copyright: {\textcopyright} 2018, Pleiades Publishing, Ltd.",

year = "2018",

month = mar,

day = "1",

doi = "10.1134/S160767291802014X",

language = "English",

volume = "479",

pages = "108--110",

journal = "Doklady Biochemistry and Biophysics",

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T1 - Translocation of Oligonucleotide–Oligosaccharide Complexes into Cells of the Brain

AU - Dygalo, N. N.

AU - Kalinina, T. S.

AU - Lanshakov, D. A.

N1 - Funding Information: We are grateful to M.V. Korobeinikov for irradiation of samples in the accelerator. This study was supported by a program of the Russian Academy of Sciences (project no. 0324-2016-0011). Publisher Copyright: © 2018, Pleiades Publishing, Ltd.

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Irradiation of a mixture of oligonucleotides with dextran resulted in the formation of a complex that is detected by a decelerated migration of an irradiated sample in electrophoretic gel compared to a non-irradiated one. When injected into the brain of neonatal rats, the formed complex penetrated into the cells 3 times easier compared to the original oligonucleotide, thus indicating that the use of radiation crosslinking of oligonucleotides with oligosaccharides is promising to enhance the efficiency of delivery of gene-targeted oligonucleotide drugs into cells.

AB - Irradiation of a mixture of oligonucleotides with dextran resulted in the formation of a complex that is detected by a decelerated migration of an irradiated sample in electrophoretic gel compared to a non-irradiated one. When injected into the brain of neonatal rats, the formed complex penetrated into the cells 3 times easier compared to the original oligonucleotide, thus indicating that the use of radiation crosslinking of oligonucleotides with oligosaccharides is promising to enhance the efficiency of delivery of gene-targeted oligonucleotide drugs into cells.

KW - Animals

KW - Biological Transport

KW - Brain/metabolism

KW - Oligonucleotides/metabolism

KW - Oligosaccharides/metabolism

KW - Rats

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JO - Doklady Biochemistry and Biophysics

JF - Doklady Biochemistry and Biophysics

SN - 1607-6729

IS - 1

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Translocation of Oligonucleotide–Oligosaccharide Complexes into Cells of the Brain

Abstract

Keywords

OECD FOS+WOS

State classification of scientific and technological information

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