Определение и сравнительный анализ геномной структуры сибирских штаммов вируса клещевого энцефалита европейского субтипа

Translated title of the contribution: Definition and comparative analysis of the genomic structure of siberian strains of tick-borne encephalitis virus of the european subtype

T. V. Demina, I. V. Kozlova, S. E. Tkachev, E. K. Doroshchenko, O. V. Lisak, Yu S. Savinova, O. V. Suntsova, M. M. Verkhozina, Yu P. Dzhloev, A. I. Paramonov, D. O. Kiselev, V. I. Zlobln

Research output: Contribution to journalArticlepeer-review

Abstract

Tick-borne encephalitis virus (TBEV) is classified into three subtypes: Far Eastern (TBEV-FE), European (TBEV-EU) and Siberian (TBEV-SIB). In Russia, these are also called genotypes 1, 2 and 3, respectively. Geographically, TBEV-EU dominates in Central and Northern Europe, but its representatives are also found to the east-along the southern part of the forest zone of extratropical Eurasia-up to Eastern Siberia and South Korea. However, the strains isolated outside Europe remain poorly investigated. In the proposed study, eight full genomes of the Siberian isolates of TBEV-EU were determined and 13 complete genomes were compared. The analysis of 152 full-genome TBEV sequences showed that the TBEV-EU has a higher degree of stability of the genome-coding region in the entire Eurasian area (3.1% of differences) compared to TBEV-FE (6.6%) and TBEV-SIB (7.8%). At the same time, the maximum differences are observed not between European and Siberian strains, as one could expect, but between the representatives from Europe-TBEV strains Mandl-2009 from Norway and Hypr from the Czech Republic. The studied strains from Siberia form the compact genetic cluster of 42 TBEV-EU strains and are divided into two subclusters-West Siberian and East Siberian variants. These variants differ in the combinations of amino acid substitutions in all proteins except NS2B. The West Siberian variant mostly circulates in the territory of Altai, and the closest relative of its representatives is Absettarov strain from the European part of Russia. The strains similar to the East Siberian variant of the European subtype were recorded in the Altai (strain 84.2, 2007) and in Belarus (N256, about 1940).

Translated title of the contributionDefinition and comparative analysis of the genomic structure of siberian strains of tick-borne encephalitis virus of the european subtype
Original languageRussian
Article number4
Pages (from-to)29-36
Number of pages8
JournalVoprosy Virusologii
Volume63
Issue number1
DOIs
Publication statusPublished - 2018
Externally publishedYes

Keywords

  • Amino acids substitutions
  • Genetic variability
  • Genotype
  • Polyprotein
  • Subtype
  • TBE
  • TBEV

State classification of scientific and technological information

  • 34.25 Virology

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