Detection of Ehrlichia spp. And Theileria spp. And Hyalomma anatolicum ticks collected in Tajikistan

M. Yu Kartashov, Yu V. Kononova, I. D. Petrova, N. L. Tupota, T. P. Mikryukova, V. A. Ternovoi, F. H. Tishkova, V. B. Loktev

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1 Citation (Scopus)

Abstract

The objectives of our study were to survey the prevalence of genetic markers for Rickettsia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., and Theileria spp. in Hyalomma anatolicum ticks collected in southwestern Tajikistan and to perform sequencing and phylogenetic analysis of fragments of the 16S rRNA gene and groESL operon from Ehrlichia spp. and fragments of the 18S rRNA gene of Theileria spp. detected in H. anatolicum ticks. Hyalomma anatolicum ticks collected in the Tursunzade and Rudaki districts of Tajikistan were tested for DNA of Rickettsia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., and Theileria spp. by PCR with specific primers. The amplified fragments were sequenced and analyzed. DNA of Ehrlichia spp. (3.3 %) and Theileria spp. (3.3 %) was detected only in H. anatolicum ticks collected from the Rudaki district, and DNA of Ehrlichia spp. (0.7 %) was found in H. anatolicum ticks from the Tursunzade district. Sequence analysis of fragments of the 16S rRNA gene and groESL operon from Ehrlichia spp. revealed high similarity to Ehrlichia spp. The Tajik isolates of Theileria spp. were genotyped as Theileria annulata based on the analysis of 18S rRNA gene sequences. The phylogenetic analysis demonstrates that Ehrlichia spp. isolates are highly similar to Ehrlichia spp. circulating in China and Brazil. The isolate Tajikistan-5 is closely related to the putative novel species Ehrlichia mineirensis. The Tajik isolates of Theileria spp. were clustered with T. annulata isolates from Turkey, Iran, Pakistan, and China by phylogenetic analyses.

Translated title of the contributionВыявление Ehrlichia spp. и Theileria spp. в клещах Hyalomma anatolicum, собранных в Таджикистане
Original languageEnglish
Pages (from-to)55-59
Number of pages5
JournalВавиловский журнал генетики и селекции
Volume24
Issue number1
DOIs
Publication statusPublished - 1 Jan 2020

Keywords

  • Ehrlichia spp.
  • Hyalomma anatolicum
  • Tajikistan
  • Theileria spp.
  • Tick-borne infections
  • TRP36
  • DNA
  • CATTLE
  • CONGO HEMORRHAGIC-FEVER
  • tick-borne infections
  • IDENTIFICATION

OECD FOS+WOS

  • 3 MEDICAL AND HEALTH SCIENCES
  • 1.06 BIOLOGICAL SCIENCES
  • 4 AGRICULTURAL SCIENCES

State classification of scientific and technological information

  • 34 BIOLOGY

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