Влияние диеты с повышенным содержанием жира на липидный профиль ооцитов мышей

Translated title of the contribution: Effects of a high-fat diet on the lipid profile of oocytes in mice

E. Yu Brusentsev, E. A. Chuyko, K. A. Okotrub, T. N. Igonina, I. N. Rozhkova, D. S. Ragaeva, S. V. Ranneva, V. A. Naprimerov, S. Ya Amstislavsky

Research output: Contribution to journalArticlepeer-review

Abstract

There are evidences that obese women exhibit a detrimental oocyte quality. However, it remains unclear how this change is associated with obesity, indirectly - or directly through a change in the content and/or composition of lipids in oocytes. The aim of this work was to study effects of a high-fat diet applied to female donor mice on the amount and qualitative composition of lipids of immature and in vivo matured oocytes. A high-fat diet caused larger body weight in female mice compared with the control (p < 0.001; 44.77 ± 1.46 and 35.22 ± 1.57, respectively), and increased the blood levels of cholesterol (p < 0.05; 2.06 ± 0.10 and 1.78 ± 0.10, respectively) and triglycerides (p < 0.05; 2.13 ± 0.23 and 1.49 ± 0.21, respectively). At the same time, this diet does not affect the level of unsaturation of lipids in immature (0.207 ± 0.004 in the experiment and 0.206 ± 0.002 in the control) and matured oocytes (0.212 ± 0.005 in the experiment and 0.211 ± 0.003 in the control). Total lipid content increased during in vivo maturation of mouse oocytes. The amount of lipids was greater in mature oocytes in the experimental group compared to the control (p < 0.01; 8.15 ± 0.37 and 5.83 ± 0.14, respectively). An increase in intracellular lipid amount during oocyte maturation was revealed both after a standard diet (p < 0.05; 4.72 ± 0.48 and 5.83 ± 0.14, respectively) and after a fat-rich diet (p < 0.001; 3.45 ± 0.62 and 8.15 ± 0.37, respectively). Thus, during in vivo oocyte maturation in mice the content of intracellular lipids enhanced, the high-fat diet aggravated this dynamics of lipid increase during in vivo maturation of oocytes.

Translated title of the contributionEffects of a high-fat diet on the lipid profile of oocytes in mice
Original languageRussian
Pages (from-to)533-538
Number of pages6
JournalВавиловский журнал генетики и селекции
Volume24
Issue number5
DOIs
Publication statusPublished - 2020

Keywords

  • mice
  • diet
  • oocytes
  • intracellular lipids
  • Nile Red
  • confocal laser scanning microscopy
  • Raman spectroscopy
  • PREIMPLANTATION EMBRYOS
  • MAMMALIAN OOCYTES
  • MOUSE OOCYTES
  • IN-VITRO
  • FERTILIZATION

OECD FOS+WOS

  • 3 MEDICAL AND HEALTH SCIENCES
  • 1.06 BIOLOGICAL SCIENCES
  • 4 AGRICULTURAL SCIENCES

State classification of scientific and technological information

  • 34 BIOLOGY

Fingerprint

Dive into the research topics of 'Effects of a high-fat diet on the lipid profile of oocytes in mice'. Together they form a unique fingerprint.

Cite this